CK1epsilon (R178C) Kinase Assay Service

Activity-based CK1epsilon (R178C) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CK1epsilon (R178C)

Enzyme

Human CK1epsilon (R178C)

HGNC

CSNK1E (R178C)

Synonym

CKIe, HCKIE, MGC10398, CK1-epsilon

Substrates

Casein, 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

P49674 (http://www.uniprot.org/uniprot/P49674)

Screening Location

Malvern, USA

Reference compound IC50 for CK1epsilon (R178C)