CK1g1 Kinase Assay Service - Reaction Biology

Activity-based CK1g1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

CK1g1

Enzyme

Human CK1g1

HGNC

CSNK1G1

Synonym

CK1-gamma1

Substrates

Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q9HCP0 (http://www.uniprot.org/uniprot/Q9HCP0)

Screening Location

Malvern, USA

Reference compound IC50 for CK1γ1