CK1g3 Kinase Assay Service

Activity-based CK1g3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CK1g3

Enzyme
Human CK1g3

HGNC
CSNK1G3

Synonym
CK1-gamma3

Substrates
Peptide substrate, [KRRRAL[pS]VASLPGL], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 4,300
GW 5074 15,000
Rottlerin 28,300
Scientific Information
Q9Y6M4 (http://www.uniprot.org/uniprot/Q9Y6M4)

Screening Location
Malvern, USA

Reference compound IC50 for CK1γ3
Reference compound IC50 for CK1γ3

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