Activity-based CK2a2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CK2a2
Enzyme
Human CK2a2
HGNC
CSNK2A2
Synonym
CK2-alpha2, Casein kinase II subunit alpha, CSK22, CSNK2A2, CK2A2
Scientific Information
P19784 (http://www.uniprot.org/uniprot/P19784)
Assay Properties
Substrates
Peptide substrate, [RRRDDDSDDD], 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 600 |
| Ro 31-8220 | 3,500 |
| GW 5074 | 180 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
| Pacritinib | 270 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for CK2a2
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