CK2a Kinase Assay Service

Activity-based CK2a kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
CK2a

Enzyme
Human CK2a

HGNC
CSNK2A1

Synonym
CKII, CK2A1, CK2-alpha1

Substrates
Peptide substrate, [RRRDDDSDDD], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1,600
Ro 31-8220 5,600
GW 5074 140
H-89 >100,000
Rottlerin >100,000
Scientific Information
P68400 (http://www.uniprot.org/uniprot/P68400)

Screening Location
Malvern, USA

Reference compound IC50 for CK2a
Reference compound IC50 for CK2a

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