Activity-based CLK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CLK1
Enzyme
Human CLK1
HGNC
CLK1
Synonym
CLK, CLK/STY
Substrates
(Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 22.6 |
| Ro 31-8220 | 13.6 |
| GW 5074 | 6,900 |
| H-89 | 13,500 |
| Rottlerin | 7,900 |
Scientific Information
P49759 (http://www.uniprot.org/uniprot/P49759)
Screening Location
Malvern, USA
Reference compound IC50 for CLK1
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