Activity-based CLK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CLK2
Enzyme
Human CLK2
HGNC
CLK2
Synonym
MGC61500, hCLK2
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 8.7 |
| Ro 31-8220 | 8.8 |
| GW 5074 | 8,200 |
| H-89 | 31,000 |
Scientific Information
P49760 (http://www.uniprot.org/uniprot/P49760)
Screening Location
Malvern, USA
Reference compound IC50 for CLK2
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