Activity-based CLK3 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
CLK3
Enzyme
Human CLK3, wt
HGNC
CLK3
Manning ID
CLK3
Synonym
clk3, CDC-like kinase 3, CLK3, PHCLK3, PHCLK3/152, CDc-like kinase 3
UniProt
P49761 (CLK3_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RB-S6P, ATP
Reaction
RB-S6P + 33P-ATP = 33P-RB-S6P + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 6733 |
| Sorafenib | 10000 |
| Sunitinib | 33000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | CLK3 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – CLK3 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0921-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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