Activity-based CLK4 (I363V) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CLK4 (I363V)
Enzyme
Human CLK4 (I363V)
HGNC
CLK4 (I363V)
Synonym
Dual specificity protein kinase CLK4, CDC-like kinase 4
Scientific Information
Q9HAZ1 (http://www.uniprot.org/uniprot/Q9HAZ1)
Assay Properties
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 16 |
| Ro 31-8220 | 6.2 |
| GW 5074 | 684 |
| H-89 | 4,100 |
| Rottlerin | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for CLK4 (I363V)
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