Activity-based CLK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
CLK4
Enzyme
Human CLK4
HGNC
CLK4
Synonym
DKFZp686A20267, CDc-like kinase 4
Substrates
MBP (Myelin Basic Protein), 10 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 10.5 |
| Ro 31-8220 | 16 |
| GW 5074 | 1,800 |
| H-89 | 7,400 |
| Rottlerin | 34,000 |
Scientific Information
Q9HAZ1 (http://www.uniprot.org/uniprot/Q9HAZ1)
Screening Location
Malvern, USA
Reference compound IC50 for CLK4
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