Activity-based COT1/MAP3K8 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
COT1/MAP3K8
Enzyme
Human COT1/MAP3K8
HGNC
MAP3K8
Synonym
COT1, EST, ESTF, TPL2, Tpl-2
Substrates
MEK1 (K97R), 1 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Compounds | IC50 (nM) |
---|---|
Ro 31-8220 | 17,000 |
GW 5074 | >20,000 |
Scientific Information
P41279 (http://www.uniprot.org/uniprot/P41279)
Screening Location
Malvern, USA
Reference compound IC50 for COT1/MAP3K8
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