COT1/MAP3K8 Kinase Assay Service

Activity-based COT1/MAP3K8 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
COT1/MAP3K8

Enzyme
Human COT1/MAP3K8

HGNC
MAP3K8

Synonym
COT1, EST, ESTF, TPL2, Tpl-2

Substrates
MEK1 (K97R), 1 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Ro 31-8220 17,000
GW 5074 >20,000
Scientific Information
P41279 (http://www.uniprot.org/uniprot/P41279)

Screening Location
Malvern, USA

Reference compound IC50 for COT1/MAP3K8
Reference compound IC50 for COT1/MAP3K8