COT1/MAP3K8 Kinase Assay Service

Activity-based COT1/MAP3K8 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

COT1/MAP3K8

Enzyme

Human COT1/MAP3K8

HGNC

MAP3K8

Synonym

COT1, EST, ESTF, TPL2, Tpl-2

Substrates

MEK1 (K97R), 1 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
Ro 31-8220	17,000
GW 5074	>20,000

Scientific Information

P41279 (http://www.uniprot.org/uniprot/P41279)

Screening Location

Malvern, USA

Reference compound IC50 for COT1/MAP3K8