Prostaglandin G/H synthase 1 (Cyclooxygenase-1, COX-1) Biochemical Activity Assay for compound screening and profiling via absorbance measurements.
Target Overview
CAT#
COX1
Target
Prostaglandin G/H synthase 1 (Cyclooxygenase-1, COX-1; ovine)
Substrate
Hemin
Scientific Information
PGH1 (https://www.uniprot.org/uniprotkb/P05979/entry)
Assay Properties
Protocol
COX1 + substrate + competing ligand incubation; add arachidonic acid and colorimetric substance and read absorbance measurement on Envision plate reader
Readout
Absorbance measurements at 590 nm (proportional to enzyme activity)
Reference Compound IC50
| Compound IC50 (nM) |
|---|
| SC-560 (27.1) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound activity for COX-1
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