Prostaglandin G/H synthase 2 (Cyclooxygenase-2, COX-2) Biochemical Activity Assay for compound screening and profiling via absorbance measurements.
Target Overview
CAT#
COX2
Target
Prostaglandin G/H synthase 2 (Cyclooxygenase-2, COX-2)
Substrate
Hemin
Scientific Information
PGH2 (https://www.uniprot.org/uniprotkb/P35354/entry)
Assay Properties
Protocol
COX2 + substrate + competing ligand incubation; add arachidonic acid and colorimetric substance and read absorbance measurement on Envision plate reader
Readout
Absorbance measurements at 590 nm (proportional to enzyme activity)
Reference Compound IC50
| Compound IC50 (nM) |
|---|
| DuP-697 (31.7) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound activity for COX-2
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