Dopaminergic D1 Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
D1 Binding
GPCR Target
Human D1
Source
Membrane preparation from CHO-K1 cells stably transfected with human dopamine D1 receptor
Scientific Information
DRD1 (https://www.uniprot.org/uniprotkb/P21728/entry)
Assay Properties
Protocol
Radioligand filter binding to D1 membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| SCH-23390 (1.8) | SCH-23390 (3) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for D1
PDF