Dopaminergic D2S Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
D2S Binding
GPCR Target
Human D2S
Source
Membrane preparation from CHO-K1 cells stably transfected with human dopamine D2S receptor
Scientific Information
F8VUV1 (https://www.uniprot.org/uniprotkb/F8VUV1/entry)
Assay Properties
Protocol
Radioligand filter binding to D2S membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| 7-OH DPAT (78) | 7-OH DPAT (15) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for D2S
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