DCAMKL2 Kinase Assay Service

Activity-based DCAMKL2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
DCAMKL2

Enzyme
Human DCAMKL2

HGNC
DCLK2

Synonym
DCDC3, DCK2, MGC45428, KIAA0369

Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 11
Ro 31-8220 5,400
GW5074 8,400
H-89 31,000
Rottlerin >100,000
Scientific Information
Q8N568 (http://www.uniprot.org/uniprot/Q8N568)

Screening Location
Malvern, USA

Reference compound IC50 for DCAMKL2
Reference compound IC50 for DCAMKL2

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