Activity-based DCAMKL3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
DCAMKL3
Enzyme
Human DCAMKL3
HGNC
DCLK3
Synonym
Serine/threonine-protein kinase DCLK3, Doublecortin domain-containing protein 3C, Doublecortin-like and CAM kinase-like 3, Doublecortin-like kinase 3, DCLK3, DCDC3C, KIAA1765
Scientific Information
Q9C098 (http://www.uniprot.org/uniprot/Q9C098)
Assay Properties
Substrates
Peptide substrate, [KKLNRTLSFAEPG], 20 µM
ATP, 10 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.6 |
| Ro 31-8220 | 110 |
| GW5074 | 4,700 |
| H-89 | 9,600 |
| Rottlerin | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for DCAMKL3
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