dDHODH Dehydrogenase Assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
dDHODH
Enzyme
Dog DHODH
Synonym
Dihydroorotate dehydrogenase (quinone) mitochondrial, Haptoglobin
Substrate
25 µM L-DHO, 100 µM resazurin, Cofactor FMN
Assay Properties
Reaction
L-DHO + FMN = Orotate + FMNH2
FMNH2 + Resazurin = FMN + Resorufin
Resorufin fluorescence, End point measurement
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| BAY-2402234 | 2.2 |
| Brequinar | 8.0 |
| Teriflunomide | 43.0 |
| Leflunomide | 372 |
Scientific Information
A0A8I3N262 (http://www.uniprot.org/uniprot/A0A8I3N262)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Nucleotide Metabolism Pathway Assays
Reference compound inhibition of dDHODH
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