Activity-based DDR2 T654M kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
DDR2 T654M
Enzyme
Human DDR2 T654M, mutant
HGNC
DDR2 (T654M)
Manning ID
DDR2
Synonym
DDR2, MIG20a, NTRKR3, TKT, TYRO10, DDR2 T654M, DDR2 (T654M)
UniProt
Q16832 (DDR2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-NTRK3tide, ATP
Reaction
RBER-NTRK3tide + 33P-ATP = 33P-RBER-NTRK3tide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 4 |
| Sorafenib | 190 |
| Sunitinib | 290 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | DDR2 T654M |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – DDR2 T654M (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1193-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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