DGKD Kinase Assay Service

Activity-based DGKD lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.

Target Overview

CAT#

DGKD

Enzyme

Human DGKD

HGNC

DGKD

Synonym

Diacylglycerol kinase delta, DAG kinase delta, DGK-delta, KIAA0145, DGKD-2

Scientific Information

Q16760 (https://www.uniprot.org/uniprot/Q16760)

Assay Properties

Substrates

DLG (1,2-Dilauroyl-sn-glycerol), 500 µM
ATP, 200 µM

Reaction

1,2-dilauroyl-sn-glycerol + ATP = 1,2-dilauroyl-sn-glycero-3-phosphate + ADP + H+

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
DGK-IN-1 (CAS No: 2407892-34-4) >100,000
Calphostin C 3,890
R-59-949 >100,000
R-59-022 >100,000
Cochlioquinone A >100,000

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for DGKD

Reference compound IC50 for DGKD