Activity-based DGKE lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
DGKE
Enzyme
Human DGKE
HGNC
DGKE
Synonym
Diacylglycerol kinase epsilon, DAG kinase epsilon, DGK-epsilon, DAGK5
Scientific Information
P52429 (https://www.uniprot.org/uniprot/P52429)
Assay Properties
Substrates
DLG (1,2-Dilauroyl-sn-glycerol), 500 µM
ATP, 200 µM
Reaction
1,2-dilauroyl-sn-glycerol + ATP = 1,2-dilauroyl-sn-glycero-3-phosphate + ADP + H+
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| DGK-IN-1 (CAS No: 2407892-34-4) | >100,000 |
| Calphostin C | 4,610 |
| R-59-949 | >100,000 |
| R-59-022 | >100,000 |
| Cochlioquinone A | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for DGKE
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