Activity-based DGKK lipid kinase assay for compound screening and profiling via ADP-Glo kinase assay.
Target Overview
CAT#
DGKK
Enzyme
Human DGKK
HGNC
DGKK
Synonym
Diacylglycerol kinase kappa, DAG kinase kappa, DGK-kappa, 142 kDa diacylglycerol kinase
Scientific Information
Q5KSL6 (https://www.uniprot.org/uniprot/Q5KSL6)
Assay Properties
Substrates
DLG (1,2-Dilauroyl-sn-glycerol), 500 µM + Phosphatidylserine, 0.1 mg/mL
ATP, 200 µM
Reaction
1,2-dilauroyl-sn-glycerol + ATP = 1,2-dilauroyl-sn-glycero-3-phosphate + ADP + H+
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| DGK-IN-1 (CAS No: 2407892-34-4) | 3,100 |
| Calphostin C | 30,100 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for DGKK
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