DNMT3B/3L Methyltransferase Assay Service

Activity-based DNMT3B/3L Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.

Target Overview

CAT#

DNMT3B/3L

Enzyme

Co-expressed Human DNMT3B/3L catalytic domain

Synonym

DNMT3B: DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB, DNM3B; DNMT3L: DNA (cytosine-5)-methyltransferase 3-like, DNM3L

Substrates

Lambda DNA, 0.0075 mg/ml
SAM, 1 µM

Assay Properties

Reaction

S-adenosyl-L-[methyl-³H]methionine + DNA = S-adenosyl-L-homocysteine + DNA 5-[methyl-³H]-cytosine

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
SAH 146
Sinefungin 210

Scientific Information

DNMT3B: Q9UBC3 (http://www.uniprot.org/uniprot/Q9UBC3)
DNMT3L: Q9UJW3 (http://www.uniprot.org/uniprot/Q9UJW3)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Methyltransferase Assays.

Reference compound IC50 for DNMT3B/3L

Reference compound IC50 for DNMT3B/3L

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