DOP (δ opioid) Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
DOP Binding
GPCR Target
Human DOP
Source
Membrane preparation from HEK293 cells stably transfected with human DOP receptor
Scientific Information
OPRD (https://www.uniprot.org/uniprotkb/P41143/entry)
Assay Properties
Protocol
Radioligand filter binding to DOP membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| DADLE (5.9) | DADLE (10) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for DOP
PDF