DOT1L Methyltransferase Assay Service

Activity-based DOT1L Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.

Target Overview

CAT#

DOT1L

Enzyme

Human DOT1L

Synonym

Histone-lysine N-methyltransferase, H3 lysine-79 specific, Histone H3-K79 methyltransferase, H3-K79-HMTase, DOT1-like protein, Lysine N-methyltransferase 4, KIAA1814, KMT4

Substrates

Oligo Nucleosomes, 0.05 mg/ml
SAM, 1 µM

Assay Properties

Reaction

S-adenosyl-L-[methyl-³H]methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N⁶-[methyl-³H]-L-lysine

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
SAH 450
Sinefungin 20,000

Scientific Information

Q8TEK3 (http://www.uniprot.org/uniprot/Q8TEK3)

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Methyltransferase Assays.

Reference compound IC50 for DOT1

Reference compound IC50 for DOT1