Activity-based DYRK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
DYRK3
Enzyme
Human DYRK3
HGNC
DYRK3
Synonym
DYRK5, RED, REDK
Substrates
Peptide substrate, [RRRFRPASPLRGPPK], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 29 |
| Ro 31-8220 | 580 |
| GW 5074 | 540 |
| H-89 | 7,000 |
| Rottlerin | >20,000 |
Scientific Information
O43781 (http://www.uniprot.org/uniprot/O43781)
Screening Location
Malvern, USA
Reference compound IC50 for DYRK3
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