Activity-based DYRK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
DYRK4
Enzyme
Human DYRK4
HGNC
DYRK4
Synonym
DYRK4
Substrates
Peptide substrate, [RRRFRPASPLRGPPK], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 18,500 |
| Ro 31-8220 | >20,000 |
| GW5074 | 11,500 |
| H-89 | >20,000 |
| Rottlerin | >20,000 |
Scientific Information
Q9NR20 (http://www.uniprot.org/uniprot/Q9NR20)
Screening Location
Malvern, USA
Reference compound IC50 for DYRK4
PDF