EDNRA Functional Cell-based Agonist Assay for compound screening and profiling via fluorescence-based quantification of intracellular calcium.
Target Overview
CAT#
EDNRA Functional Agonist
GPCR Target
Human EDNRA
Cell Line
CHO-K1/EDNRA
Scientific Information
EDNRA (https://www.uniprot.org/uniprotkb/P25101/entry)
Assay Properties
Protocol
EDNRA activation by agonist application using FLIPR Penta system cells treated with Calcium 6 dye; fluorescence kinetic measurement
Readout
Calcium 6 dye fluorescence tracking changes in intracellular calcium
Reference Compound EC50
| Compound EC50 (nM) |
|---|
| Endothelin (19.4) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound activation of EDNRA
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