EED Reader Domain Assay Service

EED Reader Domain Assay for compound screening and profiling using the HTRF assay format to measure compound binding to the target.

Target Overview

CAT#

EED-GST HTRF

Enzyme

Human Polycomb protein EED aa 2-441, GST-tagged

Synonyms

EED-GST/ b-H3K27Me3

Scientific Information

O75530 (http://www.uniprot.org/uniprot/O75530)

Assay Properties

Substrates

Biotinylated histone H3 peptide (21-44), K27Me3 (b-H3K27Me3)

Reaction

HTRF binding assay

Readout

Fluorescence

Reference Compound IC50s
Compounds IC50 (nM)
EED226 18
A395 1.3

Screening Location

Malvern, PA, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Reference Compound IC50 for EED-GST

Reference Compound IC50 for EED-GST