EED Reader Domain Assay for compound screening and profiling using the HTRF assay format to measure compound binding to the target.
Target Overview
CAT#
EED-GST HTRF
Enzyme
Human Polycomb protein EED aa 2-441, GST-tagged
Synonyms
EED-GST/ b-H3K27Me3
Scientific Information
O75530 (http://www.uniprot.org/uniprot/O75530)
Assay Properties
Substrates
Biotinylated histone H3 peptide (21-44), K27Me3 (b-H3K27Me3)
Reaction
HTRF binding assay
Readout
Fluorescence
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| EED226 | 18 |
| A395 | 1.3 |
Screening Location
Malvern, PA, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Reference Compound IC50 for EED-GST
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