EEF2K Kinase Assay Service

Activity-based EEF2K kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
EEF2K

Enzyme
Human EEF2K

HGNC
EEF2K

Synonym
HSU93850, MGC45041, eEF-2K

Substrates
Peptide substrate, [RKKFGESEKTKTKEFL], 20 µM
Ca2+-Calmodulin, 1 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine >100,000
Ro 31-8220 20,000
GW 5074 63,000
NH125 4,100
Scientific Information
O00418 (http://www.uniprot.org/uniprot/O00418)

Screening Location
Malvern, USA

Reference compound IC50 for EEF2K
Reference compound IC50 for EEF2K

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