EGFR (d747-752/P753S) Kinase Assay Service

Activity-based EGFR (d747-752/P753S) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
EGFR (d747-752/P753S)

Enzyme
Human EGFR (d747-752/P753S)

HGNC
EGFR (d747-752/P753S)

Synonym
ERBB, mENA, ERBB1

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 18
PP2 90
AG1478 <0.1
PDGFR I-III 62,000
D-64406 11,000
Scientific Information
P00533 (http://www.uniprot.org/uniprot/P00533)

Screening Location
Malvern, USA

Reference compound IC50 for EGFR (d747-752/P753S)
Reference compound IC50 for EGFR (d747-752/P753S)

PDF