EGFR (T790M/L792F/C797S/L858R) Kinase Assay Service

Activity-based EGFR (T790M/L792F/C797S/L858R) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
EGFR (T790M/L792F/C797S/L858R)

Enzyme
Human EGFR (T790M/L792F/C797S/L858R)

HGNC
EGFR (T790M/L792F/C797S/L858R)

Synonym
ERBB, mENA, ERBB1

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.5
PP2 7,500
AG1478 2,200
EGFR/ErbB Inhibitor >100,000
D-64406 5,000
Scientific Information
P00533 (http://www.uniprot.org/uniprot/P00533)

Screening Location
Malvern, USA

Reference compound IC50 for EGFR (T790M/L792F/C797S/L858R)
Reference compound IC50 for EGFR (T790M/L792F/C797S/L858R)

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