Activity-based EGFR T790M/L858R kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
EGFR T790M/L858R
Enzyme
Human EGFR T790M/L858R, mutant
HGNC
EGFR (T790M/L858R)
Manning ID
EGFR
Synonym
EGFR, ERBB, ERBB1, EGF-R T790M/L858R, mENA, EGFR T790M/L858R, EGFR (L858R/T790M)
UniProt
P00533 (EGFR_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 8 |
| Sorafenib | 10000 |
| Sunitinib | 9500 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | EGFR T790M/L858R |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 EGFR Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – EGFR T790M/L858R (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0725-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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