Activity-based EIF2AK4 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
EIF2AK4
Enzyme
Human EIF2AK4, wt
HGNC
EIF2AK4
Manning ID
GCN2
Synonym
GCN2, KIAA1338
UniProt
Q9P2K8 (E2AK4_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Other
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RB-S6P, ATP
Reaction
RB-S6P + 33P-ATP = 33P-RB-S6P + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 160 |
| Sorafenib | 10000 |
| Sunitinb | 7800 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | EIF2AK4 |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – EIF2AK4 |
| Product Number | 1504-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF