EP300-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-EP300-H
Protein
Human EP300 bromodomain, His-tagged
Synonyms
Histone acetyltransferase p300, E1A-associated protein p300, p300
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (5.8 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
40,5
Reference Compound ΔTms:
CBP112 – 6.5 (Black)SGC-CBP30 – 9 (Red)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, RVX-208, GSK2801 and PFI3)
Scientific Information
Q09472 (http://www.uniprot.org/uniprot/Q09472)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
