EPHA1 Kinase Assay Service

Activity-based EPHA1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
EPHA1

Enzyme
Human EPHA1

HGNC
EPHA1

Synonym
EPH, EPHT, EPHT1

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 115
PP2 63
AG1478 2,960
Scientific Information
P21709 (http://www.uniprot.org/uniprot/P21709)

Screening Location
Malvern, USA

Reference compound IC50 for EPHA1
Reference compound IC50 for EPHA1

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