Activity-based EPHA2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
EPHA2
Enzyme
Human EPHA2, wt
HGNC
EPHA2
Manning ID
EphA2
Synonym
ECK, EPHA2
UniProt
P29317 (EPHA2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1133 |
| Sorafenib | 1600 |
| Sunitinib | 11000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | EPHA2 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – EPHA2 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0368-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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