Activity-based EPHA4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
EPHA4
Enzyme
Human EPHA4
HGNC
EPHA4
Synonym
HEK8, SEK, TYRO1
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 7.4 |
| PP2 | 71 |
| AG1478 | 1,200 |
| D-64406 | 8,300 |
Scientific Information
P54764 (http://www.uniprot.org/uniprot/P54764)
Screening Location
Malvern, USA
Reference compound IC50 for EPHA4
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