EPHA8 Kinase Assay Service

Activity-based EPHA8 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
EPHA8

Enzyme
Human EPHA8

HGNC
EPHA8

Synonym
EEK, HEK3, KIAA1459

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.1 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 64.7
PP2 66.8
AG1478 3,900
Scientific Information
P29322 (http://www.uniprot.org/uniprot/P29322)

Screening Location
Malvern, USA

Reference compound IC50 for EPHA8
Reference compound IC50 for EPHA8

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