EPHB3 Kinase Assay Service

Activity-based EPHB3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
EPHB3

Enzyme
Human EPHB3

HGNC
EPHB3

Synonym
ETK2, HEK2, TYRO6

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 825
PP2 107
AG1478 30,000
Scientific Information
P54753 (http://www.uniprot.org/uniprot/P54753)

Screening Location
Malvern, USA

Reference compound IC50 for EPHB3
Reference compound IC50 for EPHB3

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