Activity-based EPHB4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
EPHB4
Enzyme
Human EPHB4
HGNC
EPHB4
Synonym
HTK, MYK1, TYRO11
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 112 |
| PP2 | 96 |
| AG1478 | 700 |
| D-64406 | 13,000 |
Scientific Information
P54760 (http://www.uniprot.org/uniprot/P54760)
Screening Location
Malvern, USA
Reference compound IC50 for EPHB4
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