Activity-based ERBB2 775YVMA776 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
ERBB2 775YVMA776
Enzyme
Human ERBB2 775YVMA776, mutant
HGNC
ERBB2 (775YVMA776)
Manning ID
HER2/ErbB2
Synonym
ERBB2, CD340, HER2, MLN19, NGL, TKR1, ERBB2 775YVMA776
UniProt
P04626 (ERBB2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 825 |
| Sorafenib | 10000 |
| Sunitinib | 71000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | ERBB2 775YVMA776 |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – ERBB2 775YVMA776 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1525-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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