Activity-based ERBB2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
ERBB2
Enzyme
Human ERBB2, wt
HGNC
ERBB2
Manning ID
HER2/ErbB2
Synonym
NEU, NGL, HER2, TKR1, HER-2, ERBB2, ERBB2 wt, ERBB2/HER2
UniProt
P04626 (ERBB2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 450 |
| Sorafenib | 10000 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | ERBB2 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – ERBB2 |
| Product Number | 0108-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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