ERBB2 (V777_G778insCG) Kinase Assay Service

Activity-based ERBB2 (V777_G778insCG) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
ERBB2 (V777-G778insCG)

Enzyme
Human ERBB2 (V777-G778insCG)

HGNC
ERBB2 (V777_G778insCG)

Synonym
NEU, NGL, HER2, TKR1

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 140
PP2 25
AG1478 3.7
PDGFR I-III >100,000
D-64406 39,000
Scientific Information
P04626 (http://www.uniprot.org/uniprot/P04626)

Screening Location
Malvern, USA

Reference compound IC50 for ERBB2 (V777-G778insCG)
Reference compound IC50 for ERBB2 (V777-G778insCG)

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