ERBB2 (V956R) Kinase Assay Service

Activity-based ERBB2 (V956R) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview

CAT#

ERBB2 (V956R)

Enzyme

Human ERBB2 (V956R)

HGNC

ERBB2 (V956R)

Synonym

Receptor tyrosine-protein kinase erbB-2 [Precursor], p185erbB2, C-erbB-2, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, MLN 19

Scientific Information

P04626 (http://www.uniprot.org/uniprot/P04626)

Assay Properties

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/mL
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 620
PP2 510
AG1478 120
EGFR/Erb Inhibitor 0.8
D-64406 >100,000
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for ERBB2 (V956R)