Activity-based ERBB4/HER4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ErbB4
Enzyme
Human ErbB4
HGNC
ERBB4
Synonym
HER4
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.1 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 96.7 |
| PP2 | 274 |
| AG1478 | 29.6 |
| D-64406 | 15,000 |
Scientific Information
Q15303 (http://www.uniprot.org/uniprot/Q15303)
Screening Location
Malvern, USA
Reference compound IC50 for ErbB4/HER4
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