Activity-based ERK2/MAPK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ERK2/MAPK1
Enzyme
Human ERK2/MAPK1
HGNC
MAPK1
Synonym
ERK2, ERT1, P42MAPK, PRKM1,
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 790 |
| Ro 31-8220 | 21,000 |
| GW 5074 | >100,000 |
| SCH772984 | 0.5 |
| LY3214996 | 0.2 |
Scientific Information
P28482 (http://www.uniprot.org/uniprot/P28482)
Screening Location
Malvern, USA
Reference compound IC50 for ERK2/MAPK1
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