Activity-based ERK5 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
ERK5
Enzyme
Human ERK5, wt
HGNC
MAPK7 (aa5-397)
Manning ID
Erk5
Synonym
BMK1, ERK4, ERK5, PRKM7, BMK1 kinase, MAPK7, ERK5/MAPK7 CD
UniProt
Q13164 (MK07_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 50 |
| Sorafenib | 10000 |
| Sunitinb | 6000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | ERK5 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – ERK5 |
| Product Number | 1105-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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