Activity-based ERK5/MAPK7 FL kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ERK5/MAPK7
Enzyme
Human ERK5/MAPK7
HGNC
MAPK7
Synonym
BMK1, PRKM7
Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 10,000 |
| Ro 31-8220 | 49,000 |
| GW5074 | 9,100 |
| H-89 | >50,000 |
| Rottlerin | >50,000 |
Scientific Information
Q13164 (http://www.uniprot.org/uniprot/Q13164)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for ERK5/MAPK7
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