Activity-based FAK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
FAK
Enzyme
Human FAK, wt
HGNC
PTK2
Manning ID
FAK
Synonym
FADK, FAK1, pp125FAK, PTK2, FAK aa2-1052, FAK, FAK/PTK2
UniProt
Q05397 (FAK1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 22 |
| Sorafenib | 10000 |
| Sunitinib | 900 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | FAK |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – FAK (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0165-0000-3 |
| Web Shop | Reaction Biology Online Shop |
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